A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
Jash B, Scharf P, Sandmann N, Fonseca Guerra C, Megger DA, Müller J
Research article (journal) | Peer reviewedAbstract
A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag+-mediated base pair between P and cytosine, whereas the presence of Ag+ strongly destabilizes nucleic acids comprising an artificial base pair between P and thymine. Metal-mediated base pair formation was investigated by temperature-dependent UV spectroscopy and CD spectroscopy and complemented by extensive DFT calculations. The molecular beacon significantly extends the application spectrum of nucleic acids with metal-mediated base pairs. It is of potential use in the detection of single-nucleotide polymorphisms.
Details about the publication
Authors from the University of Münster
| Müller, Jens | |
| Sandmann, Nikolas | |
| Scharf, Philipp |
Projects the publication originates from
Duration: 01/01/2010 - 31/12/2017 | 2nd Funding period Funded by: DFG - Collaborative Research Centre Type of project: Subproject in DFG-joint project hosted at University of Münster |
Doctorates the publication originates from
| 1H-Imidazo[4,5-f][1,10]phenanthroline as a ligand in metal-modified nucleic acids Candidate: Jash, Biswarup | Supervisors: Müller, Jens Period of time: 01/10/2014 - 14/02/2018 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |