A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
Jash B, Scharf P, Sandmann N, Fonseca Guerra C, Megger DA, Müller J
Forschungsartikel (Zeitschrift) | Peer reviewedZusammenfassung
A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag+-mediated base pair between P and cytosine, whereas the presence of Ag+ strongly destabilizes nucleic acids comprising an artificial base pair between P and thymine. Metal-mediated base pair formation was investigated by temperature-dependent UV spectroscopy and CD spectroscopy and complemented by extensive DFT calculations. The molecular beacon significantly extends the application spectrum of nucleic acids with metal-mediated base pairs. It is of potential use in the detection of single-nucleotide polymorphisms.
Details zur Publikation
Autor*innen der Universität Münster
| Müller, Jens | Professur für Anorganische Chemie (Prof. Müller) |
| Sandmann, Nikolas | Professur für Anorganische Chemie (Prof. Müller) |
| Scharf, Philipp | Institut für Anorganische und Analytische Chemie |
Projekte, aus denen die Publikation entstanden ist
Laufzeit: 01.01.2010 - 31.12.2017 | 2. Förderperiode Gefördert durch: DFG - Sonderforschungsbereich Art des Projekts: Teilprojekt in DFG-Verbund koordiniert an der Universität Münster |
Promotionen, aus denen die Publikation resultiert
| 1H-Imidazo[4,5-f][1,10]phenanthroline as a ligand in metal-modified nucleic acids Promovend*in: Jash, Biswarup | Betreuer*innen: Müller, Jens Zeitraum: 01.10.2014 - 14.02.2018 Promotionsverfahren erfolgt(e) an: Promotionsverfahren an der Universität Münster |