Application of a Metal-Mediated Base Pair to the Detection of Medicinally Relevant Single Nucleotide Polymorphisms
Jash B, Müller J
Research article (journal) | Peer reviewedAbstract
A highly stabilizing metal-mediated base pair formed from the canonical nucleobase cytosine (C), the transition metal ion AgI, and the artificial nucleobase 1H-imidazo[4,5-f][1,10]phenanthroline (P) was used in the generation of molecular beacons for the detection of medicinally relevant single nucleotide polymorphisms (SNPs) based on either a C→T or a T→C transition (T: thymine). The beacons exploit the fact that a P–C pair is strongly stabilized in the presence of AgI, whereas a P–T pair is significantly destabilized. A direct correlation of oligonucleotide sequence, duplex stability prior to the addition of AgI, stabilization due to metal-mediated base pair formation, and performance of the molecular beacon was not observed. Nonetheless, the data show for the first time that metal-mediated base pairing can be applied in the detection of medicinally relevant SNPs.
Details about the publication
Authors from the University of Münster
| Jash, Biswarup | Professorship of Inorganic Chemistry (Prof. Müller) |
| Müller, Jens | Professorship of Inorganic Chemistry (Prof. Müller) |
Projects the publication originates from
Duration: 01/01/2010 - 31/12/2017 | 2nd Funding period Funded by: DFG - Collaborative Research Centre Type of project: Subproject in DFG-joint project hosted at University of Münster |
Promotionen, aus denen die Publikation resultiert
| 1H-Imidazo[4,5-f][1,10]phenanthroline as a ligand in metal-modified nucleic acids Candidate: Jash, Biswarup | Supervisors: Müller, Jens Period of time: 01/10/2014 - 14/02/2018 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |