Laser ablation low-flow ICP-MS for elemental bioimaging
Steingrobe T., Niehoff A., Franze B., Lenhard D., Pietsch H., Engelhard C., Karst U., Buscher W.
Research article (journal) | Peer reviewedAbstract
A laser ablation system (LA) was coupled to an in-house developed low-flow inductively coupled plasma ion source for mass spectrometry (ICP-MS). In this setup, the low-flow torch showed best analytical performance at a total argon gas flow rate of only 1.27 L min-1 and a generator power of 900 W. The optimized system was applied to elemental bioimaging. Two different sections of one kidney sample were analyzed and images of the elements Al and Br from the staining agents hematoxylin and eosin, respectively, and I from a contrast agent iodixanol were recorded selectively and with high sensitivity. For detection of the elements, the mass traces 27Al, 79Br, 81Br, and 127I were selected to demonstrate the system's analytical performance over a wide mass range. In total, the argon consumption could be reduced by >90% compared to conventional ICP-MS systems.
Details about the publication
Authors from the University of Münster
| Buscher, Wolfgang | Professur für Analytische Chemie (Prof. Karst) |
| Karst, Uwe | Professur für Analytische Chemie (Prof. Karst) |
Promotionen, aus denen die Publikation resultiert
| Elemental and Molecular Mass Spectrometry for Complementary Bioimaging: From Method Development to Biomedical Applications Candidate: Niehoff, Ann-Christin (geb. Bülter) | Supervisors: Karst, Uwe Period of time: 01/04/2013 - 02/03/2016 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster | |
| Entwicklung einer großflächigen Glimmentladungsquelle und eines Laserablations-Niedrigfluss-ICP-MS-Systems als neuartige elementselektive Imaging-Techniken Candidate: Steingrobe, Tobias | Supervisors: Karst, Uwe; Buscher, Wolfgang; Hayen, Heiko Period of time: 01/10/2012 - 31/10/2015 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |