Laser ablation low-flow ICP-MS for elemental bioimaging
Steingrobe T., Niehoff A., Franze B., Lenhard D., Pietsch H., Engelhard C., Karst U., Buscher W.
Forschungsartikel (Zeitschrift) | Peer reviewedZusammenfassung
A laser ablation system (LA) was coupled to an in-house developed low-flow inductively coupled plasma ion source for mass spectrometry (ICP-MS). In this setup, the low-flow torch showed best analytical performance at a total argon gas flow rate of only 1.27 L min-1 and a generator power of 900 W. The optimized system was applied to elemental bioimaging. Two different sections of one kidney sample were analyzed and images of the elements Al and Br from the staining agents hematoxylin and eosin, respectively, and I from a contrast agent iodixanol were recorded selectively and with high sensitivity. For detection of the elements, the mass traces 27Al, 79Br, 81Br, and 127I were selected to demonstrate the system's analytical performance over a wide mass range. In total, the argon consumption could be reduced by >90% compared to conventional ICP-MS systems.
Details zur Publikation
Autor*innen der Universität Münster
| Buscher, Wolfgang | Professur für Analytische Chemie (Prof. Karst) |
| Karst, Uwe | Professur für Analytische Chemie (Prof. Karst) |
Promotionen, aus denen die Publikation resultiert
| Elemental and Molecular Mass Spectrometry for Complementary Bioimaging: From Method Development to Biomedical Applications Promovend*in: Niehoff, Ann-Christin (geb. Bülter) | Betreuer*innen: Karst, Uwe Zeitraum: 01.04.2013 - 02.03.2016 Promotionsverfahren erfolgt(e) an: Promotionsverfahren an der Universität Münster | |
| Entwicklung einer großflächigen Glimmentladungsquelle und eines Laserablations-Niedrigfluss-ICP-MS-Systems als neuartige elementselektive Imaging-Techniken Promovend*in: Steingrobe, Tobias | Betreuer*innen: Karst, Uwe; Buscher, Wolfgang; Hayen, Heiko Zeitraum: 01.10.2012 - 31.10.2015 Promotionsverfahren erfolgt(e) an: Promotionsverfahren an der Universität Münster |