Quantitative bioimaging of platinum group elements in tumor spheroids
Niehoff, A.; Grünebaum, J.; Moosmann, A.; Mulac, D.; Söbbing, J.; Niehaus, R.; Buchholz, R.; Kröger, S.; Wiehe, A.; Wagner, S.; Sperling, M.; von Briesen, H.; Langer, K.; Karst, U.
Research article (journal) | Peer reviewedAbstract
Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
Details about the publication
Authors from the University of Münster
| Buchholz, Rebecca | |
| Grünebaum, Jonas | |
| Karst, Uwe | |
| Kröger, Sabrina | |
| Langer, Klaus | |
| Mulac-Hahnen, Dennis | |
| Niehaus, Rebecca | |
| Söbbing, Judith | |
| Sperling, Michael |
Doctorates the publication originates from
| LA-ICP-MS Systems for Bioimaging: Towards Representative Sampling, Fast Washout, High Spatial Resolution and Sensitive Detection of Organic Aerosols Candidate: Niehaus, Rebecca | Supervisors: Karst, Uwe Period of time: 01/10/2012 - 31/10/2015 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |