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Quantitative bioimaging of platinum group elements in tumor spheroids

Niehoff, A.; Grünebaum, J.; Moosmann, A.; Mulac, D.; Söbbing, J.; Niehaus, R.; Buchholz, R.; Kröger, S.; Wiehe, A.; Wagner, S.; Sperling, M.; von Briesen, H.; Langer, K.; Karst, U.

Research article (journal) | Peer reviewed

Abstract

Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.

Details about the publication

Journal: Analytica Chimica Acta
Volume: 938
Page range: 106-113
Status: Published
Release year: 2016
Language in which the publication is written: English
DOI: 10.1016/j.aca.2016.07.021
Link to the full text: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84995564848&origin=inward
Keywords: LA-ICP-MS; Photosensitizer; Pt cytostatics; Quantification; Tumor spheroids

Authors from the University of Münster

Buchholz, Rebecca
Professur für Analytische Chemie (Prof. Karst)
Grünebaum, Jonas
Institute for Pharmaceutical Biology and Phytochemistry
Karst, Uwe
Professur für Analytische Chemie (Prof. Karst)
Kröger, Sabrina
Professur für Analytische Chemie (Prof. Karst)
Langer, Klaus
Professorship of Pharmaceutical Technology and Biopharmacy (Prof. Langer)
Mulac-Hahnen, Dennis
Professorship of Pharmaceutical Technology and Biopharmacy (Prof. Langer)
Niehaus, Rebecca
Institute of Physical Chemistry
Söbbing, Judith
Professorship of Pharmaceutical Technology and Biopharmacy (Prof. Langer)
Sperling, Michael
Professur für Analytische Chemie (Prof. Karst)

Promotionen, aus denen die Publikation resultiert

LA-ICP-MS Systems for Bioimaging: Towards Representative Sampling, Fast Washout, High Spatial Resolution and Sensitive Detection of Organic Aerosols
Candidate: Niehaus, Rebecca | Supervisors: Karst, Uwe
Period of time: 01/10/2012 - 31/10/2015
Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster