Quantitative bioimaging of platinum group elements in tumor spheroids
Niehoff A., Grünebaum J., Moosmann A., Mulac D., Söbbing J., Niehaus R., Buchholz R., Kröger S., Wiehe A., Wagner S., Sperling M., von Briesen H., Langer K., Karst U.
Research article (journal) | Peer reviewedAbstract
Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
Details about the publication
Authors from the University of Münster
| Buchholz, Rebecca | Professur für Analytische Chemie (Prof. Karst) |
| Grünebaum, Jonas | Institute for Pharmaceutical Biology and Phytochemistry |
| Karst, Uwe | Professur für Analytische Chemie (Prof. Karst) |
| Kröger, Sabrina | Professur für Analytische Chemie (Prof. Karst) |
| Langer, Klaus | Professorship of Pharmaceutical Technology and Biopharmacy (Prof. Langer) |
| Mulac-Hahnen, Dennis | Professorship of Pharmaceutical Technology and Biopharmacy (Prof. Langer) |
| Niehaus, Rebecca | Institute of Physical Chemistry |
| Söbbing, Judith | Professorship of Pharmaceutical Technology and Biopharmacy (Prof. Langer) |
| Sperling, Michael | Professur für Analytische Chemie (Prof. Karst) |
Promotionen, aus denen die Publikation resultiert
| LA-ICP-MS Systems for Bioimaging: Towards Representative Sampling, Fast Washout, High Spatial Resolution and Sensitive Detection of Organic Aerosols Candidate: Niehaus, Rebecca | Supervisors: Karst, Uwe Period of time: 01/10/2012 - 31/10/2015 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |