Quantitative bioimaging of platinum group elements in tumor spheroids
Niehoff, A.; Grünebaum, J.; Moosmann, A.; Mulac, D.; Söbbing, J.; Niehaus, R.; Buchholz, R.; Kröger, S.; Wiehe, A.; Wagner, S.; Sperling, M.; von Briesen, H.; Langer, K.; Karst, U.
Forschungsartikel (Zeitschrift) | Peer reviewedZusammenfassung
Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
Details zur Publikation
Autor*innen der Universität Münster
| Buchholz, Rebecca | Professur für Analytische Chemie (Prof. Karst) |
| Grünebaum, Jonas | Institut für Pharmazeutische Biologie und Phytochemie |
| Karst, Uwe | Professur für Analytische Chemie (Prof. Karst) |
| Kröger, Sabrina | Professur für Analytische Chemie (Prof. Karst) |
| Langer, Klaus | Professur für Pharmazeutische Technologie und Biopharmazie (Prof. Langer) |
| Mulac-Hahnen, Dennis | Professur für Pharmazeutische Technologie und Biopharmazie (Prof. Langer) |
| Niehaus, Rebecca | Institut für Physikalische Chemie |
| Söbbing, Judith | Professur für Pharmazeutische Technologie und Biopharmazie (Prof. Langer) |
| Sperling, Michael | Professur für Analytische Chemie (Prof. Karst) |
Promotionen, aus denen die Publikation resultiert
| LA-ICP-MS Systems for Bioimaging: Towards Representative Sampling, Fast Washout, High Spatial Resolution and Sensitive Detection of Organic Aerosols Promovend*in: Niehaus, Rebecca | Betreuer*innen: Karst, Uwe Zeitraum: 01.10.2012 - 31.10.2015 Promotionsverfahren erfolgt(e) an: Promotionsverfahren an der Universität Münster |