A Dinuclear Mercury(II)-Mediated Base Pair in DNA

Mandal S, Hebenbrock M, Müller J

Research article (journal) | Peer reviewed

Abstract

The first dinuclear metal-mediated base pair containing divalent metal ions has been prepared. A combination of the neutral bis(monodentate) purine derivative 1,N6-ethenoadenine (ϵA), which preferentially binds two metal ions with a parallel alignment of the N−M bonds, and the canonical nucleobase thymine (T), which readily deprotonates in the presence of HgII and thereby partially compensates the charge accumulation due to the two closely spaced divalent metal ions, yields the dinuclear T-HgII2-ϵA base pair. This metal-mediated base pair stabilizes the DNA oligonucleotide duplex as shown by an increase of 8 °C in its melting temperature. Formation of the base pair was demonstrated by temperature-dependent UV spectroscopy as well as by titration experiments monitored by UV and CD spectroscopy.

Details about the publication

Journal: Angewandte Chemie International Edition (Angew. Chem. Int. Ed.)

Volume: 55

Page range: 15520-15523

Status: Published

Release year: 2016 (15/11/2016)

Language in which the publication is written: English

DOI: 10.1002/anie.201608354

Link to the full text: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85000642925&origin=inward

Keywords: bioinorganic chemistry; ethenoadenine; mercury; metal bioconjugates; metal-mediated base pair

Authors from the University of Münster

Hebenbrock, Marian	Professorship of Inorganic Chemistry (Prof. Müller)
Mandal, Soham	Professorship of Inorganic Chemistry (Prof. Müller)
Müller, Jens	Professorship of Inorganic Chemistry (Prof. Müller)

Projects the publication originates from

CRC 858 B09 - Influence of consecutive metal-mediated base pairs in DNA double helices on charge transfer through DNA and other cooperative effects in metal… (SFB 858) Duration: 01/01/2010 - 31/12/2017 \| 2nd Funding period Funded by: DFG - Collaborative Research Centre Type of project: Subproject in DFG-joint project hosted at University of Münster
NRW Research School "Molecules and Materials – A Common Design Principle" (Molecules and Materials) Duration: 01/10/2009 - 31/10/2012 Funded by: Federal Ministry of Culture and Science of the Federal State of North Rhine-Westphalia Type of project: Individual project

Promotionen, aus denen die Publikation resultiert

Metal-Mediated Base Pairs with Ethenoadenine, a Versatile Nucleobase Surrogate
Candidate: Mandal, Soham | Supervisors: Müller, Jens
Period of time: 08/10/2012 - 16/08/2016
Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster