Complexation and oxidation strategies for improved TXRF determination of mercury in vaccines
Holtkamp M, Elseberg T, Wehe CA, Sperling M, Karst U
Research article (journal) | Peer reviewedAbstract
A thorough investigation of strategies to overcome the effect of mercury losses during TXRF analysis was carried out. The vaporisation of mercury on sampling targets{,} associated with too low concentrations determined{,} depends on the nature of the respective Hg species and the dwell time of the analytes on the target. To prevent vaporisation{,} oxidation with ammonium persulfate and complexation with ethylenediamine tetraacetate (EDTA) and dimercaptosuccinic acid (DMSA) were investigated. Whereas both approaches were effective in reducing the Hg losses by vaporisation{,} the complexation approaches turned out to be particularly efficient. Both EDTA and DMSA retain the different mercury species quantitatively over several hours. Based on this approach{,} a method for mercury determination by TXRF in liquid samples was developed{,} successfully validated by inductively coupled plasma optical emission spectrometry and applied to the analysis of a mercury-containing vaccine sample.
Details about the publication
Authors from the University of Münster
| Elseberg, Tim | |
| Holtkamp, Michael | |
| Karst, Uwe |
Doctorates the publication originates from
| Laser Ablation-Ambient Ionization-Mass Spectrometry and Micro X-Ray Fluorescence Imaging as Orthogonal Techniques for the Analysis of Pharmaceuticals Candidate: Elseberg, Tim | Supervisors: Karst, Uwe Period of time: 01/04/2013 - 17/10/2017 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster | |
| Pushing inductively coupled-mass spectrometry to its limits: Towards improved sensitivity, robustness, speed and sample consumption Candidate: Wehe, Christoph Alexander | Supervisors: Karst, Uwe; Schwerdtle, Tanja Period of time: 01/01/2011 - 31/03/2014 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |