Complexation and oxidation strategies for improved TXRF determination of mercury in vaccines

Holtkamp M, Elseberg T, Wehe CA, Sperling M, Karst U

Zusammenfassung

A thorough investigation of strategies to overcome the effect of mercury losses during TXRF analysis was carried out. The vaporisation of mercury on sampling targets{,} associated with too low concentrations determined{,} depends on the nature of the respective Hg species and the dwell time of the analytes on the target. To prevent vaporisation{,} oxidation with ammonium persulfate and complexation with ethylenediamine tetraacetate (EDTA) and dimercaptosuccinic acid (DMSA) were investigated. Whereas both approaches were effective in reducing the Hg losses by vaporisation{,} the complexation approaches turned out to be particularly efficient. Both EDTA and DMSA retain the different mercury species quantitatively over several hours. Based on this approach{,} a method for mercury determination by TXRF in liquid samples was developed{,} successfully validated by inductively coupled plasma optical emission spectrometry and applied to the analysis of a mercury-containing vaccine sample.

Promotionen, aus denen die Publikation resultiert