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Alpha2-adrenergic receptors and their core involvement in the process of axonal growth in retinal explants.

Prokosch V, Panagis L, Volk GF, Dermon C, Thanos S

Research article (journal)

Abstract

To determine the patterns of ?2-adrenergic receptor (?2-AR) subtype expression in normal and degenerated retinas and to analyze the response of these receptors to the ?2-AR agonist brimonidine tartrate (BT).The binding characteristics of ?2-ARs in the retina were evaluated in experimental and matching sham groups by in vitro quantitative autoradiographic saturation with [(3)H]-clonidine. Retinal explants from juvenile and adult rats with either elevated intraocular pressure or after optic nerve crush (ONC) were cultured with BT over 96 hours in vitro to analyze the effects of BT on axonal growth by videomicroscopy and axon counting. Changes in retinal protein expression by BT were monitored by two-dimensional gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS).The total number of ?2-ARs in the retina increased significantly after ONC compared with the sham group. BT supported axonal growth in the juvenile, glaucomatous, and injured retinas (P < 0.004) most effectively at a concentration of 0.001 mg/mL, without influencing the axonal growth rate. Immediate supplementation of BT was more effective than delayed supplementation (P < 0.001). Proteomic analysis revealed treatment-specific expression patterns of glial fibrillary acidic protein (GFAP), glucose-related protein (GRP)58, platelet-activating factor (PAF), and laminin-binding protein (LBP).These data are the first to show differences in ?2-AR expression in normal and degenerated retinas. BT supports neuronal growth in cultured retinal pieces, suggesting that ?2-ARs play a role in retinal metabolism.

Details about the publication

Journal: Investigative Ophthalmology & Visual Science (Invest Ophthalmol Vis Sci)
Volume: 51
Issue: 12
Page range: 6688-6699
Status: Published
Release year: 2010
Language in which the publication is written: English
Keywords: Optic Nerve Injuries; Retina; Electrophoresis Gel Two-Dimensional; Rats; Animals; Retinal Degeneration; Adrenergic alpha-2 Receptor Agonists; Rats Sprague-Dawley; Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization; Quinoxalines; Platelet Activating Factor; Disease Models Animal; Autoradiography; Glaucoma; Organ Culture Techniques; Receptors Laminin; Protein Disulfide-Isomerases; Proteomics; Fluorescent Antibody Technique Indirect; Axons; Receptors Adrenergic alpha-2. Glial Fibrillary Acidic Protein; Clonidine; Optic Nerve Injuries; Retina; Electrophoresis Gel Two-Dimensional; Rats; Animals; Retinal Degeneration; Adrenergic alpha-2 Receptor Agonists; Rats Sprague-Dawley; Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization; Quinoxalines; Platelet Activating Factor; Disease Models Animal; Autoradiography; Glaucoma; Organ Culture Techniques; Receptors Laminin; Protein Disulfide-Isomerases; Proteomics; Fluorescent Antibody Technique Indirect; Axons; Receptors Adrenergic alpha-2. Glial Fibrillary Acidic Protein; Clonidine

Authors from the University of Münster

Prokosch, Verena
Clinic for Ophthalmology
Thanos, Solon
Institut für Experimentelle Ophthalmologie

Distinctions received for the publication

Glaukomforschungspreis der DOG
Awarded by: Deutsche Ophthalmologische Gesellschaft (DOG)
Award given to: Prokosch, Verena; Fuchshofer, Rudolf
Date of awarding: 15/06/2010
Type of distinction: Research award or other distinction