Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm.
Mellmann A, Weniger T, Berssenbrügge C, Keckevoet U, Friedrich AW, Harmsen D, Grundmann H
Research article (journal)Abstract
We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).
Details about the publication
Journal: Journal of Clinical Microbiology (J Clin Microbiol)
Volume: 46
Issue: 8
Page range: 2805-2808
Status: Published
Release year: 2008
Language in which the publication is written: English
DOI: 10.1128/JCM.00071-08
Keywords: Humans; DNA Bacterial; Sequence Analysis DNA. Staphylococcus aureus; Cluster Analysis; Staphylococcal Protein A. Bacterial Typing Techniques; Electrophoresis Gel Pulsed-Field; Genotype; Humans; DNA Bacterial; Sequence Analysis DNA. Staphylococcus aureus; Cluster Analysis; Staphylococcal Protein A. Bacterial Typing Techniques; Electrophoresis Gel Pulsed-Field; Genotype
Authors from the University of Münster
| Berssenbrügge, Christoph | Medical Clinic of Internal Medicine B: Gastroenterology and Metabolic Disorders (Med B) |
| Harmsen, Dag | Department of Periodontology |
| Mellmann, Alexander | Institute of Hygiene |