Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm.

Mellmann A, Weniger T, Berssenbrügge C, Keckevoet U, Friedrich AW, Harmsen D, Grundmann H

Research article (journal)

Abstract

We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).

Details about the publication

JournalJournal of Clinical Microbiology (J Clin Microbiol)
Volume46
Issue8
Page range2805-2808
StatusPublished
Release year2008
Language in which the publication is writtenEnglish
DOI10.1128/JCM.00071-08
KeywordsHumans; DNA Bacterial; Sequence Analysis DNA. Staphylococcus aureus; Cluster Analysis; Staphylococcal Protein A. Bacterial Typing Techniques; Electrophoresis Gel Pulsed-Field; Genotype; Humans; DNA Bacterial; Sequence Analysis DNA. Staphylococcus aureus; Cluster Analysis; Staphylococcal Protein A. Bacterial Typing Techniques; Electrophoresis Gel Pulsed-Field; Genotype

Authors from the University of Münster

Berssenbrügge, Christoph
Medical Clinic of Internal Medicine B: Gastroenterology and Metabolic Disorders (Med B)
Harmsen, Dag
Department of Periodontology
Mellmann, Alexander
Institute of Hygiene