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Generation of readily transformable Bacillus licheniformis mutants.

Waschkau B, Waldeck J, Wieland S, Eichstädt R, Meinhardt F

Research article (journal) | Peer reviewed

Abstract

A set of mutants was generated by targeted deletion of the hsdR loci of two type I restriction modification systems (RMS) identified in Bacillus licheniformis DSM13. Single as well as double knock-outs resulted in strains being readily transformable with plasmids isolated from Bacilli. Introduction of shuttle plasmids isolated from Escherichia coli was routinely possible when the double mutant B. licheniformis MW3 (DeltahsdR1, DeltahsdR2) was used in transformation experiments. Growth and secretion of extracellular enzymes were not affected in any of the mutants. Thus, along with an optimized transformation protocol, this study makes available an urgently needed transformation system for this industrially exploited species.

Details about the publication

Journal: Applied Microbiology and Biotechnology
Volume: 78
Issue: 1
Page range: 181-188
Status: Published
Release year: 2008
Language in which the publication is written: English
Keywords: Genetic Vectors; DNA Bacterial; Mutation; Transformation Bacterial; DNA Restriction-Modification Enzymes; Plasmids; Bacillus; Escherichia coli; Gene Deletion; Genetic Vectors; DNA Bacterial; Mutation; Transformation Bacterial; DNA Restriction-Modification Enzymes; Plasmids; Bacillus; Escherichia coli; Gene Deletion

Authors from the University of Münster

Meinhardt, Friedhelm
Professur für Molekulare Mikrobiologie und Biotechnologie (Prof. Meinhardt)

Projects the publication originates from

Improvement of Bacillus strains for the production of extracelluar enzymes
Duration: 26/11/2008 - 31/05/2012
Funded by: Wirtschaft
Type of project: Individual project