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Generation of readily transformable Bacillus licheniformis mutants.

Waschkau B, Waldeck J, Wieland S, Eichstädt R, Meinhardt F

Forschungsartikel (Zeitschrift) | Peer reviewed

Zusammenfassung

A set of mutants was generated by targeted deletion of the hsdR loci of two type I restriction modification systems (RMS) identified in Bacillus licheniformis DSM13. Single as well as double knock-outs resulted in strains being readily transformable with plasmids isolated from Bacilli. Introduction of shuttle plasmids isolated from Escherichia coli was routinely possible when the double mutant B. licheniformis MW3 (DeltahsdR1, DeltahsdR2) was used in transformation experiments. Growth and secretion of extracellular enzymes were not affected in any of the mutants. Thus, along with an optimized transformation protocol, this study makes available an urgently needed transformation system for this industrially exploited species.

Details zur Publikation

Fachzeitschrift: Applied Microbiology and Biotechnology
Jahrgang / Bandnr. / Volume: 78
Ausgabe / Heftnr. / Issue: 1
Seitenbereich: 181-188
Status: Veröffentlicht
Veröffentlichungsjahr: 2008
Sprache, in der die Publikation verfasst ist: Englisch
Stichwörter: Genetic Vectors; DNA Bacterial; Mutation; Transformation Bacterial; DNA Restriction-Modification Enzymes; Plasmids; Bacillus; Escherichia coli; Gene Deletion; Genetic Vectors; DNA Bacterial; Mutation; Transformation Bacterial; DNA Restriction-Modification Enzymes; Plasmids; Bacillus; Escherichia coli; Gene Deletion

Autor*innen der Universität Münster

Meinhardt, Friedhelm
Professur für Molekulare Mikrobiologie und Biotechnologie (Prof. Meinhardt)

Projekte, aus denen die Publikation entstanden ist

Verbesserung von Bacillus Stämmen zur Produktion extrazellulärer Enzyme
Laufzeit: 26.11.2008 - 31.05.2012
Gefördert durch: Wirtschaft
Art des Projekts: Gefördertes Einzelprojekt