CRC 1348 - A02: Local signaling for tight coupling of synaptic exo- and endocytosis
Basic data for this project
Type of project: Subproject in DFG-joint project hosted at University of Münster
Duration at the University of Münster: 01/01/2026 - 31/12/2029 | 3rd Funding period
Description
At the synaptic cellular interface compensatory endocytosis after synaptic vesicle exocytosis is crucial to maintain a pool of release-ready vesicles at predefined release sites. We use our xenapse culture system in conjunction with total internal reflection fluorescence microscopy (TIRFM) and CRISPR-Cas9 genome editing to visualize individual exocytosis and endocytosis events and study the function of release sites and endocytic membrane domains. Cryo-electron tomography of xenapses grown on TEM grids is utilized to correlate function with structure.
Keywords: Biologie; zelluläre Kontaktstellen; Bildgebung
Website of the project: https://www.uni-muenster.de/SFB1348/en/projects/PROJECT_A02.html
DFG-Gepris-ID: https://gepris.dfg.de/gepris/projekt/393717959
Funding identifier: SFB 1348/3, A02 | DFG project number: 386797833
Funder / funding scheme:
- DFG - Collaborative Research Centre (SFB)
Project management at the University of Münster
| Klingauf, Jürgen | Institute of Medical Physics and Biophysics |
Applicants from the University of Münster
| Klingauf, Jürgen | Institute of Medical Physics and Biophysics |