CRC 1348 - A02: Local signaling for tight coupling of synaptic exo- and endocytosis

Basic data for this project

Type of project: Subproject in DFG-joint project hosted at University of Münster

Duration at the University of Münster: 01/01/2026 - 31/12/2029 | 3rd Funding period

Description

At the synaptic cellular interface compensatory endocytosis after synaptic vesicle exocytosis is crucial to maintain a pool of release-ready vesicles at predefined release sites. We use our xenapse culture system in conjunction with total internal reflection fluorescence microscopy (TIRFM) and CRISPR-Cas9 genome editing to visualize individual exocytosis and endocytosis events and study the function of release sites and endocytic membrane domains. Cryo-electron tomography of xenapses grown on TEM grids is utilized to correlate function with structure.

Keywords: Biologie; zelluläre Kontaktstellen; Bildgebung

Website of the project: https://www.uni-muenster.de/SFB1348/en/projects/PROJECT_A02.html

DFG-Gepris-ID: https://gepris.dfg.de/gepris/projekt/393717959

Funding identifier: SFB 1348/3, A02 | DFG project number: 386797833

Funder / funding scheme: