Molecular Cloning and Characterization of Rubber Biosynthetic Genes from Taraxacum koksaghyz

Schmidt T, Hillebrand A, Wurbs D, Wahler D, Lenders M, Gronover CS, Pruefer D

Forschungsartikel (Zeitschrift) | Peer reviewed

Zusammenfassung

Rubber biosynthesis requires the action of specific enzymes known as cis-prenyltransferases (CPTs). These enzymes are responsible for the sequential addition of isopentenyl pyrophosphate units to the growing polyisoprene chain, a biochemical reaction thought to be stimulated by the presence of small rubber particle proteins (SRPPs). We have cloned, characterized, and analyzed the expression of three CPT genes (TkCPT1-3) and five SRPP genes (TkSRPP1-5) from the rubber-producing plant Taraxacum koksaghyz. The deduced TkCPT amino acid sequences showed significant levels of sequence identity with Hevea brasiliensis CPTs. We also found no obvious differences between SRPPs from T. koksaghyz, another rubber producer, and a non-rubber plant. The roles of the individual TkCPTs and TkSRRPs in rubber biosynthesis are discussed. © Springer-Verlag 2009. Language of original document

Details zur Publikation

FachzeitschriftPlant Molecular Biology Reporter
Jahrgang / Bandnr. / Volume28
Ausgabe / Heftnr. / Issue2
Seitenbereich277-284
StatusVeröffentlicht
Veröffentlichungsjahr2010
Sprache, in der die Publikation verfasst istEnglisch
DOI10.1007/s11105-009-0145-9
StichwörterLatex; Laticifers; Rubber; Rubber particles; Taraxacum koksaghyz

Autor*innen der Universität Münster

Hillebrand, Andrea
Plant Biotechnology (AG Prof. Prüfer)
Lenders, Malte
Plant Biotechnology (AG Prof. Prüfer)
Prüfer, Dirk
Plant Biotechnology (AG Prof. Prüfer)
Schulze Gronover, Christian
Plant Biotechnology (AG Prof. Prüfer)