Acetan-like heteropolysaccharide production by various Kozakia baliensis strains: Characterization and further insights

Julia Wünsche, Hannah Brüggemann, Moritz Gansbiller, Jochen Schmid

Abstract

The family of Acetobacteraceae has demonstrated their ability to produce several heteropolysaccharides with a strong structural resemblance to xanthan gum. In this study, we assessed the potential of three isolates of K. baliensis as exopolysaccharide producers, namely K. baliensis SR-745, K. baliensis LMG 27018, and K. baliensis SR-1290. Among these, K. baliensis SR-745 was identified as the most promising candidate, exhibiting a final exopolysaccharide titer of 7.09 (± 0.50) g∙L−1 and a productivity of 0.15 (± 0.01) g∙L−1∙h−1. Subsequent monomer analysis confirmed structural variations for the side chain composition of different strains. A molar subunit ratio of 6:1:1:1 (d-glucose: D-mannose: D-galactose: D-glucuronic acid) for EPS derived from K. baliensis SR-745 and of 3:1:3:1 for K. baliensis LMG 27018 was determined, while the exopolysaccharide produced by K. baliensis SR-1290 consisted of a major share of rhamnose. In-depth rheological polymer characterizations revealed high viscosity rates and predominantly elastic gel character, making polysaccharides of K. baliensis highly interesting for applications in the food and cosmetic industry. Further insights into the fundamental structure-function relationships of biopolymers were obtained by comparing exopolysaccharides derived from K. baliensis to a genetically engineered xanthan variant lacking acetyl and pyruvyl substitutions.