Simvastatin Induces In Vitro Mineralization Effects of Primary Human Odontoblast-Like Cells.

Sabandal, MMI; Schäfer, E; Imper, J; Jung, S; Kleinheinz, J; Sielker, S

Research article (journal) | Peer reviewed

Abstract

Simvastatin (SV) is an often prescribed statin reducing the LDL-concentration in circulating blood. The aim of this study was to evaluate the pleiotropic effects of SV to primary human odontoblast-like cells. Twenty four wisdom teeth of different subjects were extracted and the pulp tissue was removed and minced under sterile conditions. After mincing, the requested cells were passaged according to established protocols. Osteoblastic marker (ALP conversion), viability and mineralization were determined at days 14, 17 and 21 after simvastatin exposition (0.01 µM, 0.1 µM, 1.0 µM, 2.0 µM). The sample size per group was 24 cultures with three replicates per culture for ALP-conversion and mineralization and 6 replicates for viability. A Kruskal–Wallis test was used for statistical analysis. After adding SV, viability was significantly (p < 0.01) decreased in a time- and dose-dependent manner, whereas after 21 days, mineralization was significant (p < 0.01). ALP-conversion in groups with SV concentrations of 1 and 2 µM SV was significantly (p < 0.01) increased. Pleiotropic effects regarding mineralization in higher SV concentrations were possibly induced via alternative mineralization pathways as almost equal elevations of ALP conversion were not evident in the control and experimental groups.

Details about the publication

JournalMaterials
Volume13
Issue20
StatusPublished
Release year2020
Language in which the publication is writtenEnglish
DOI10.3390/ma13204679
Link to the full texthttps://www.mdpi.com/1996-1944/13/20/4679
Keywordsmineralization; odontoblast-like cells; pleiotropic effects; simvastatin

Authors from the University of Münster

Jung, Susanne
Clinic for Cranio-Maxillofacial Surgery
Kleinheinz, Johannes
Clinic for Cranio-Maxillofacial Surgery
Sabandal, Martin
Department of Operative and Preventive Dentistry
Schäfer, Edgar
Zentrale Interdisziplinäre Ambulanz in der ZMK
Sielker, Sonja
Clinic for Cranio-Maxillofacial Surgery