Negative Ion-Mode N-Glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS
Soltwisch J.; Heijs B.
Research article (journal) | Peer reviewedAbstract
Matrix-assisted laser desorption/ionization mass spectrometry imaging with laser-induced postionization (MALDI-2-MSI) has proven a powerful tool for the in situ analysis of N-linked glycosylation, or N-glycans, directly from clinical tissue samples. Here we describe a sample preparation protocol for the analysis of N-glycans from formalin-fixed, paraffin-embedded tissue sections.
Details about the publication
Journal: Methods in Molecular Biology
Volume: 2688
Page range: 173-186
Status: Published
Release year: 2023 (07/07/2023)
Language in which the publication is written: English
Link to the full text: https://api.elsevier.com/content/abstract/scopus_id/85164247241
Keywords: Laser-induced postionization; MALDI-2; Mass spectrometry imaging; Matrix-assisted laser desorption/ionization; N-Linked glycosylation; Oligosaccharides; On-tissue enzymatic digestion
Authors from the University of Münster
| Soltwisch, Jens | Institute of Hygiene |