A Ca2+-sensor switch for tolerance to elevated salt stress in Arabidopsis

Steinhorst L.; He G.; Moore L.K.; Schültke S.; Schmitz-Thom I.; Cao Y.; Andrés Z.; Hashimoto K.; Ragel P.; Piepenburg K.; Almutairi B.O.; Behera S.; Wyganowski T.; Batistič O.; Edel K.H.; Köster P.; Krebs M.; Zhang C.; Guo Y.; Jiang C.; Bock R.; Quintero F.J.; Kudla J.

Abstract

Excessive Na+ in soils inhibits plant growth. Here, we report that Na+ stress triggers primary calcium signals specifically in a cell group within the root differentiation zone, thus forming a “sodium-sensing niche” in Arabidopsis. The amplitude of this primary calcium signal and the speed of the resulting Ca2+ wave dose-dependently increase with rising Na+ concentrations, thus providing quantitative information about the stress intensity encountered. We also delineate a Ca2+-sensing mechanism that measures the stress intensity in order to mount appropriate salt detoxification responses. This is mediated by a Ca2+-sensor-switch mechanism, in which the sensors SOS3/CBL4 and CBL8 are activated by distinct Ca2+-signal amplitudes. Although the SOS3/CBL4-SOS2/CIPK24-SOS1 axis confers basal salt tolerance, the CBL8-SOS2/CIPK24-SOS1 module becomes additionally activated only in response to severe salt stress. Thus, Ca2+-mediated translation of Na+ stress intensity into SOS1 Na+/H+ antiporter activity facilitates fine tuning of the sodium extrusion capacity for optimized salt-stress tolerance.