The Use of Long-Read Sequencing Technologies in Infection Control: Horizontal Transfer of a bla CTX-M-27 Containing lncFII Plasmid in a Patient Screening Sample

Almsick V, Schuler F, Mellmann A, Schwierzeck V

Abstract

Abstract Plasmid transfer is one important mechanism how antimicrobial resistance can spread between different species, contributing to the rise of multidrug resistant bacteria (MDRB) worldwide. Here were present whole genome sequencing (WGS) data of two MDRB isolates, anEscherichia coliand aKlebsiella quasipneumoniae,which were isolated from a single patient. Detailed analysis of long-read sequencing data identified an identical F2:A-:B- lncFII plasmid containingblaCTX-M-27in both isolates, suggesting horizontal plasmid exchange between the two species. As the plasmid of theE. colistrain carried multiple copies of the resistance cassette, the genomic data correlated with the increased antimicrobial resistance (AMR) detected for this isolate. Our case report demonstrates how long-read sequencing data of MDRB can be used to investigate the role of plasmid mediate resistance in the healthcare setting and explain resistance phenotypes.