Technology or ecology? New tools to assess cognitive judgement bias in mice

Krakenberg V, Woigk I, Garcia Rodriguez L, Kästner N, Kaiser S, Sachser N, Richter SH

Abstract

Cognitive judgement bias tests have become important new tools for the assessment of animal emotions. They allow for the inference of an animal's emotional state based on ambiguous cue interpretations. As mice are the predominantly used animal model for cognitive and behavioural neuroscience, research in this field would considerably benefit from the development of suitable judgement bias tests for this species. Against this background, we aimed to implement two different active choice cognitive judgement bias paradigms for mice in a methodological study. For this purpose, two experiments were conducted: in experiment I, an automated, vision-based touchscreen technique was applied, allowing for the direct translation of tasks from rodents to humans and vice versa. Experiment II comprised a task relying on more ecologically relevant cues in form of tunnels of different lengths. While the touchscreen task was characterized by automation-related advantages such as the possibility to present many trials per session and a high convenience for the experimenter, the tunnel task was learned faster by the mice. In both tests, however, the response to the trained and ambiguous conditions resulted in a graded curve, the basic requirement for proving task validity. Thus, both the translational touchscreen task as well as the ecologically more relevant tunnel task could successfully be implemented and provide new tools for the future assessment of cognitive judgement biases in mice.