Characterisation and cellular uptake of polysorbate 80-coated PLA nanoparticles

Raudszus B., Partikel K., Mulac D., Langer K.

Abstract

The blood brain-barrier is a major obstacle in the treatment of several central nervous disorders. In this context polysorbate 80-coated nanoparticles, which are quite well characterised concerning their behaviour in vivo, are an often described approach to enable transport of certain drugs into the brain. In the present study we prepared polysorbate 80-coated nanoparticles based on poly(lactid acid) and quantified the amount of adsorbed polysorbate 80 spectrophotometrically. In order to receive information about the behaviour in biological systems we analysed the protein corona by SDS-PAGE and finally investigated the cellular uptake in dependence of a previous incubation with fetal bovine serum.