Elemental bioimaging of manganese uptake in C. elegans
Große Brinkhaus S., Bornhorst J., Chakraborty S., Wehe C., Niehaus R., Reifschneider O., Aschner M., Karst U.
Research article (journal) | Peer reviewedAbstract
A new method for elemental bioimaging with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed and applied to study the uptake of manganese (Mn) in Caenorhabditis elegans (C. elegans). C. elegans is a well-established model organism in neuroscience, genetics and genomics, which has been extensively studied to decipher mechanisms of heavy metal induced toxicity. Knowledge about the distribution of manganese (Mn) and other metals in this organism will be helpful in elucidating pathways and mechanisms of transport, distribution and excretion. The LA-ICP-MS method requires limited sample preparation and can be used rapidly and easily to visualize the Mn distribution in C. elegans. Due to thorough optimization of the analytical parameters, intense Mn signals in C. elegans wild-type (WT) and mutants were obtained at a spatial resolution as small as 4 μm, thus proving the suitability of LA-ICP-MS to study the uptake of metals in C. elegans. © 2014 The Royal Society of Chemistry.
Details about the publication
Authors from the University of Münster
| Große Brinkhaus, Sigrid | Professur für Angewandte Geologie (Prof. Achten) |
| Karst, Uwe | Professur für Analytische Chemie (Prof. Karst) |
| Niehaus, Rebecca | Professur für Analytische Chemie (Prof. Karst) |
| Reifschneider, Olga | Institute of Inorganic and Analytical Chemistry |
| Wehe, Christoph | Professur für Analytische Chemie (Prof. Karst) |
Promotionen, aus denen die Publikation resultiert
| LA-ICP-MS Systems for Bioimaging: Towards Representative Sampling, Fast Washout, High Spatial Resolution and Sensitive Detection of Organic Aerosols Candidate: Niehaus, Rebecca | Supervisors: Karst, Uwe Period of time: 01/10/2012 - 31/10/2015 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster | |
| Pushing inductively coupled-mass spectrometry to its limits: Towards improved sensitivity, robustness, speed and sample consumption Candidate: Wehe, Christoph Alexander | Supervisors: Karst, Uwe; Schwerdtle, Tanja Period of time: 01/01/2011 - 31/03/2014 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |