Phase 1 and 2 drug metabolites generated using a miniaturized electrochemical cell with an attached ESI needle

Van Den Brink F., Büter L., Odijk M., Olthuis W., Karst U., Van Den Berg A.

Abstract

In this work we show phase 1 and 2 drug metabolism using a miniaturized electrochemical cell followed by on-line metabolite detection using electrospray ionization - mass spectrometry (ESI-MS). The most common form of in vivo drug metabolism is enzyme-catalyzed oxidation [1]. Therefore, a method to rapidly mimic these reactions and subsequently analyze the products is interesting for drug screening applications. With the versatile experimental setup presented, phase 1 metabolism of clozapine is carried out with a very short transfer time to detection, as well as phase 1 and 2 metabolism of paracetamol which is first oxidized and subsequently conjugated to glutathione. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.