Characterization of KIBRA, a novel WW domain-containing protein

Kremerskothen J.,Plaas C.,Büther K.,Finger I.,Veltel S.,Matanis T.,Liedtke T.,Barnekow A.,

Research article (journal) | Peer reviewed

Abstract

In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein. © 2002 Elsevier Science (USA). All rights reserved.

Details about the publication

JournalBiochemical and Biophysical Research Communications (Biochem Biophys Res Commun)
Volume300
Issue4
Page range867null
StatusPublished
Release year2003 (24/01/2003)
Language in which the publication is writtenEnglish
DOI10.1016/S0006-291X(02)02945-5

Authors from the University of Münster

Schwegmann, Katrin
European Institute of Molecular Imaging (EIMI)