Imaging by Elemental and Molecular Mass Spectrometry Reveals the Uptake of an Arsenolipid in the Brain of Drosophila melanogaster

Niehoff A., Schulz J., Soltwisch J., Meyer S., Kettling H., Sperling M., Jeibmann A., Dreisewerd K., Francesconi K., Schwerdtle T., Karst U.

Research article (journal) | Peer reviewed

Abstract

Arsenic-containing lipids (arsenolipids) are natural products of marine organisms such as fish, invertebrates, and algae, many of which are important seafoods. A major group of arsenolipids, namely, the arsenic-containing hydrocarbons (AsHC), have recently been shown to be cytotoxic to human liver and bladder cells, a result that has stimulated interest in the chemistry and toxicology of these compounds. In this study, elemental laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) and molecular matrix-assisted laser desorption/ionization (MALDI-)MS were used to image and quantify the uptake of an AsHC in the model organism Drosophila melanogaster. Using these two complementary methods, both an enrichment of arsenic and the presence of the AsHC in the brain were revealed, indicating that the intact arsenolipid had crossed the blood-brain barrier. Simultaneous acquisition of quantitative elemental concentrations and molecular distributions could allow new insight into organ-specific enrichment and possible transportation processes of arsenic-containing bioactive compounds in living organisms.

Details about the publication

JournalAnalytical Chemistry (Anal Chem)
Volume88
Issue10
Page range5258-5263
StatusPublished
Release year2016 (17/05/2016)
Language in which the publication is writtenEnglish
DOI10.1021/acs.analchem.6b00333
Link to the full texthttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84969776793&origin=inward
KeywordsARSENIC-CONTAINING HYDROCARBONS; LIPIDS; OIL; RESOLUTION; MODEL; FISH

Authors from the University of Münster

Dreisewerd, Klaus
Institute of Hygiene
Jeibmann, Astrid
Institute of Neuropathology
Karst, Uwe
Professur für Analytische Chemie (Prof. Karst)
Meyer, Sören
Institute of Food Chemistry
Schulz, Jacqueline
Institute of Neuropathology
Soltwisch, Jens
Institute of Hygiene
Sperling, Michael
Professur für Analytische Chemie (Prof. Karst)