Quantification of Manganese Enhanced Magnetic Resonance Imaging based on Spatially Resolved Elemental Mass Spectrometry
Niehoff AC , Wachsmuth L , Schmid F , Sperling M, Faber C , Karst U
Research article (journal) | Peer reviewedAbstract
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is proposed as a method to calibrate manganese enhanced magnetic resonance imaging (MEMRI) for the quantitative analysis of manganese accumulation in rat brain. T-1 maps of the brain of rats, either naive or after systemic fractionated manganese chloride administration were recorded in vivo and brain slices were subsequently investigated by LA-ICP-MS. Based on the proportional relationship between R-1, the rate constant of T-1 relaxation, and the concentration of manganese, a conversion function for retrospective quantification was generated, using a region of interest (ROI) analysis of corresponding MEMRI and LA-ICP-MS images. This conversion function was used to transform in vivo T-1 maps directly into local manganese concentrations, thus allowing an externally validated quantification of MEMRI in vivo experiments for the first time.
Details about the publication
Authors from the University of Münster
| Karst, Uwe | |
| Schmid, Florian | |
| Sperling, Michael | |
| Wachsmuth, Lydia |
Doctorates the publication originates from
| Elemental and Molecular Mass Spectrometry for Complementary Bioimaging: From Method Development to Biomedical Applications Candidate: Niehoff, Ann-Christin (geb. Bülter) | Supervisors: Karst, Uwe Period of time: 01/04/2013 - 02/03/2016 Doctoral examination procedure finished at: Doctoral examination procedure at University of Münster |