Murine endoscopy for in vivo multimodal imaging of carcinogenesis and assessment of intestinal wound healing and inflammation

Brückner M., Lenz P., Nowacki T., Pott F., Foell D., Bettenworth D.

Research article (journal) | Peer reviewed

Abstract

Mouse models are widely used to study pathogenesis of human diseases and to evaluate diagnostic procedures as well as therapeutic interventions preclinically. However, valid assessment of pathological alterations often requires histological analysis, and when performed ex vivo, necessitates death of the animal. Therefore in conventional experimental settings, intra-individual follow-up examinations are rarely possible. Thus, development of murine endoscopy in live mice enables investigators for the first time to both directly visualize the gastrointestinal mucosa and also repeat the procedure to monitor for alterations. Numerous applications for in vivo murine endoscopy exist, including studying intestinal inflammation or wound healing, obtaining mucosal biopsies repeatedly, and to locally administer diagnostic or therapeutic agents using miniature injection catheters. Most recently, molecular imaging has extended diagnostic imaging modalities allowing specific detection of distinct target molecules using specific photoprobes. In conclusion, murine endoscopy has emerged as a novel cutting-edge technology for diagnostic experimental in vivo imaging and may significantly impact on preclinical research in various fields.

Details about the publication

JournalJournal of Visualized Experiments
Volumenull
Issue90
StatusPublished
Release year2014
Language in which the publication is writtenEnglish
DOI10.3791/51875
Link to the full texthttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84906761407&origin=inward
KeywordsCarcinogenesis; Diagnostic imaging; Experimental colitis; Gastroenterology; In vivo imaging; Intestinal wound healing; Issue 90; Medicine; Murine endoscopy

Authors from the University of Münster

Bettenworth, Dominik
FB05 - Faculty of Medicine (FB05)
Brückner, Markus Georg
Medical Clinic of Internal Medicine B: Gastroenterology and Metabolic Disorders (Med B)
Lenz, Philipp
Medical Clinic of Internal Medicine B: Gastroenterology and Metabolic Disorders (Med B)