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A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups.

Paneto GG, Köhnemann S, Martins JA, Cicarelli RM, Pfeiffer H

Research article (journal) | Peer reviewed

Abstract

SNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction, in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. Complete SNP profiles were obtained from 10 pg of total DNA. We conclude that it is possible to build and genotype more than forty mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations.

Details about the publication

Journal: Mitochondrion
Volume: 11
Issue: 2
Page range: 296-302
Status: Published
Release year: 2011
Language in which the publication is written: English
Keywords: Haplotypes; DNA Primers; Humans; Polymorphism Single Nucleotide; Brazil; Polymerase Chain Reaction; Base Sequence; DNA Mitochondrial; Reproducibility of Results; Forensic Genetics; Haplotypes; DNA Primers; Humans; Polymorphism Single Nucleotide; Brazil; Polymerase Chain Reaction; Base Sequence; DNA Mitochondrial; Reproducibility of Results; Forensic Genetics

Authors from the University of Münster

Köhnemann, Stephan
Institute of Forensic Medicine
Pfeiffer, Heidi
Institute of Forensic Medicine