Establishment of Cellobiose Utilization for Lipid Production in Rhodococcus opacus PD630.

Hetzler S, Steinbüchel A

Abstract

Rhodococcus opacus PD630, which is known for its ability to accumulate large amounts of triacylglycerols (TAG), was metabolically engineered, and a cellobiose utilization pathway was introduced. Activities of β-glucosidases were determined, and recombinant strains accumulated fatty acids up to 39.5 ± 5.7% (wt/wt) of cell dry mass from cellobiose.