High-resolution imaging of cytoplasmic Ca2+ dynamics in Arabidopsis roots.
Behera S, Kudla J
Research article (journal) | Peer reviewedAbstract
This protocol describes a method for imaging cytoplasmic Ca(2+) dynamics in roots with high resolution using confocal laser scanning microscopy (CLSM). The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, stably expressed in plants under the control of the ubiquitin promoter UBQ10, is used for Ca(2+) measurements. This protocol enables imaging of 5- to 7-d-old seedlings with high-magnification objectives (25×, 40×, and 63×).
Details about the publication
Journal: Cold Spring Harbor Protocols
Volume: 2013
Issue: 7
Status: Published
Release year: 2013 (30/07/2013)
Language in which the publication is written: English
Authors from the University of Münster
| Behera, Smrutisanjita | Institute of Plant Biology and Biotechnology |
| Kudla, Jörg | Molecular Genetics and Cell Biology of Plants - Group Prof. Jörg Kudla |