Live cell imaging of cytoplasmic Ca2+ dynamics in Arabidopsis guard cells.

Behera S, Kudla J

Research article (journal) | Peer reviewed

Abstract

This protocol describes a classical method for measuring cytoplasmic Ca(2+) dynamics in Arabidopsis thaliana guard cells. The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, under the control of the guard cell-specific promoter GC1, is used for Ca(2+) measurements.

Details about the publication

JournalCold Spring Harbor Protocols
Volume2013
Issue7
Page range665-9
StatusPublished
Release year2013 (30/07/2013)
Language in which the publication is writtenEnglish
DOI10.1101/pdb.prot072983

Authors from the University of Münster

Behera, Smrutisanjita
Institute of Plant Biology and Biotechnology
Kudla, Jörg
Molecular Genetics and Cell Biology of Plants - Group Prof. Jörg Kudla