Boost and loss of immune responses against tumor-associated antigens in the course of pregnancy as a model for allogeneic immunotherapy

Lutz M, Worschech A, Alb M, Gahn S, Bernhard L, Schwab M, Obermeier S, Einsele H, Kämmerer U, Heuschmann P, Klinker E, Otto C, Mielke S

Research article (journal) | Peer reviewed

Abstract

Donor-derived immunity against tumor-associated antigens (TAAs) may exert selective antileukemic activity reprieving the allogeneic recipient from graft-versus-host disease. As TAAs are highly expressed in placental tissues we hypothesized that pregnancy could drive respective immunity in healthy individuals. Thus,weinvestigated the frequency and level of immune responses against clinically relevant TAAs in 114 blood donors and 44 women during their first pregnancy. Quantitative reverse-transcription polymerase chain reaction was employed to detect low levels of interferon-γ after primary peptide stimulation of CD8+ T lymphocytes. In blood donors, primary immune responses of low and/or high avidity were found against WT1(15%), MUC1(14%), PRAME(7%), and HER2/neu (5%) and exerted killing functions against leukemic cells.Menhadhigher responses thanwomen,likely due to gonadal cancer-testis-antigen expression. Interestingly, a history of prior deliverywasnot associated with increased responses, whereasthe strongest responses during pregnancy werefound in early trimesters to disappear after delivery. This boost and loss of TAA-specific immunity suggests that virtually every donor harbors the potential to mount antileukemic immune responses in a recipient. However, in the absence of the driving target and a permissive environment, they are short-lived and thus require supplemental strategies such as vaccination or immunomodulation to facilitate their persistence. © 2015 by The American Society of Hematology.

Details about the publication

JournalBlood (Blood)
Volume125
Issue2
Page range261-272
StatusPublished
Release year2015 (08/01/2015)
Language in which the publication is writtenEnglish
DOI10.1182/blood-2014-09-601302

Authors from the University of Münster

Lutz, Mathias
Medical Clinic of Internal Medicine A (Hematology, Oncology, and Oneumology) (Med A)