Pre-analytical in-vitro stability of [-2]proPSA in blood and serum.

Semjonow A, Köpke T, Eltze E, Pepping-Schefers B, Bürgel H, Darte C

Research article (journal)

Abstract

[-2]proPSA may discriminate prostate cancer from benign biopsy results. We characterized the pre-analytical stability of [-2]proPSA.22 volunteers, total PSA of 4.5-19.3microg/L, had blood drawn simultaneously. Baseline measurements were performed and samples were stored under various conditions prior to measurements. Freeze-thaw cycles were performed. [-2]proPSA was measured with the p2PSA automated research use only immunoassay on the Access analyzer.Mean [-2]proPSA increases with clotting time, exceeding 10% change in recovery after 3h. In serum, [-2]proPSA values decline over time under investigated storage conditions. Serum samples kept frozen show less than 10% variation in recoveries over the course of 2 freeze-thaw cycles.For proper measurement of [-2]proPSA, blood samples should be centrifuged within 3h of blood draw. Serum may be stored at RT or refrigerated (+4 degrees C) for a maximum of 48h and should be frozen if stored for a longer period. Two freeze-thaw cycles have no effect on [-2]proPSA stability.

Details about the publication

JournalClinical Biochemistry (Clin Biochem)
Volume43
Issue10-11
Page range926-928
StatusPublished
Release year2010
Language in which the publication is writtenEnglish
KeywordsMale; Humans; Predictive Value of Tests; Reference Values; Blood Specimen Collection; Prostate-Specific Antigen; Blood Preservation; Immunoassay; Linear Models; Male; Humans; Predictive Value of Tests; Reference Values; Blood Specimen Collection; Prostate-Specific Antigen; Blood Preservation; Immunoassay; Linear Models

Authors from the University of Münster

Köpke, Thomas
Clinic for Urology
Semjonow, Axel
Clinic for Urology