Pichia acaciae killer system: Genetic analysis of toxin immunity
Paluszynski JP, Klassen R, Meinhardt F
Research article (journal) | Peer reviewedAbstract
The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPacl-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells.
Details about the publication
Journal: Applied and Environmental Microbiology (Appl. Environ. Microbiol.)
Volume: 73
Issue: 13
Page range: 4373-4378
Status: Published
Release year: 2007 (31/07/2007)
Language in which the publication is written: English
DOI: 10.1128/AEM.00271-07
Keywords: kluyveromyces-lactis zymocin deoxyribonucleic-acid plasmids linear dna plasmids saccharomyces-cerevisiae yeast expression identification toxicity inositovora promoters
Authors from the University of Münster
| Meinhardt, Friedhelm |
Projects the publication originates from
Duration: 01/09/2005 - 31/08/2008 Funded by: DFG - Individual Grants Programme Type of project: Individual project | |
Plasmid- encoded yeast killer systems (linear plasmids) Duration: since 01/01/2000 Type of project: Own resources project |