A modified DNA isolation protocol for obtaining pure RT-PCR grade RNA
Klassen R, Fricke J, Pfeiffer A, Meinhardt F
Research article (journal) | Peer reviewedAbstract
We provide a simple but very efficient method for RNA preparation from Saccharomyces cerevisiae based on a standard chromosomal DNA isolation protocol. The method yields DNA-free total RNA, including mRNA, rRNA, and tRNA but can easily be adjusted to considerably enrich low molecular weight RNAs, such as tRNAs and the small rRNA species (5S and 5.8S). The procedure was proven and validated by verification of cDNAs belonging to four different genes, two of which encoding polypeptides and two tRNA genes. Besides its simpleness, the method is further advantagous in terms of safety (omitting hazardous phenol) and cost efficiency.
Details about the publication
Journal: Biotechnology Letters
Volume: 30
Issue: 6
Page range: 1041-1044
Status: Published
Release year: 2008 (30/06/2008)
Language in which the publication is written: English
Keywords: RT-PCR RNA isolation yeast saccharomyces-cerevisiae enzymatic amplification alcoholic fermentation sequences diagnosis
Authors from the University of Münster
| Meinhardt, Friedhelm |
Projects the publication originates from
Duration: 01/09/2005 - 31/08/2008 Funded by: DFG - Individual Grants Programme Type of project: Individual project | |
Plasmid- encoded yeast killer systems (linear plasmids) Duration: since 01/01/2000 Type of project: Own resources project |