Fluorescence Integrated Single-Cell Analysis Script (FISCAS) - A tool for efficient generation of single-cell spectra FISCAS — ein Werkzeug zur effizienten Erzeugung von Einzelzellspektren

Schwenzfeier J.; Soltwisch J.

Abstract

We introduce a Fluorescence Integrated Single-Cell Analysis Script (FISCAS) that combines fluorescence microscopy with MALDI-MSI to streamline single-cell analysis. FISCAS enables automated selection of tight measurement regions, thereby reducing the acquisition of off-target pixels, and makes use of established algorithms for cell segmentation and coregistration to rapidly compile single-cell mass spectra.