Electrochemistry and LC-MS for Metabolite Generation and Identification: Tools, Technologies, and Trends
Lohmann W, Baumann A, Karst U
Research article (journal)Abstract
The elucidation of oxidative metabolism reactions is a crucial point in drug development. Generally, in vivo or in vitro experiments with liver microsomes or hepatocytes are performed. A complementary, purely instrumental method is presented in this article. In an electrochemical (EC) flow-through cell, the oxidative metabolism of xenobiotics is simulated. The cell is used in an online set-up with liquid chromatography-mass spectrometry (LC-MS), so that a separation with subsequent detection and identification of the metabolites is possible. The article provides an introduction to this new approach. The instrumental setup is presented and selected applications of EC-LC-MS in drug development processes are discussed.
Details about the publication
Authors from the University of Münster
| Baumann, Anne | Institute of Inorganic and Analytical Chemistry |
| Karst, Uwe | Professur für Analytische Chemie (Prof. Karst) |