CRC 1009 B10 - The role of calcium mediated actin rearrangement (CaAR) in podocyte function

Basic data for this project

Type of project: Subproject in DFG-joint project hosted at University of Münster

Duration at the University of Münster: 01/07/2016 - 30/06/2020 | 1st Funding period

Description

We have identified a fundamental ability of mammalian cells to reset their actin cytoskeleton in response to calcium influx (Calcium mediated Actin Rearrangement - CaAR). Calcium, actin and a key regulator of CaAR, the formin INF2, have been implicated in injury of podocytes, the cells responsible for ultrafiltration in the kidney. In this proposal we therefore want to establish the molecular basis and physiological relevance of CaAR for the function of podocytes. We will use a combination of live cell imaging and genetic perturbations in cultured cells and mouse models, with a particular focus on the role and biochemical interactions of INF2.

Keywords: Cell biology; clinical nephrology; Cell Imaging

DFG-Gepris-ID: https://gepris.dfg.de/gepris/projekt/320811840

Funding identifier: SFB 1009/2 | DFG project number: 194468054

Funder / funding scheme:

DFG - Collaborative Research Centre (SFB)

Project management at the University of Münster

Pavenstädt, Hermann-Joseph	Medical Clinic of Internal Medicine D (Nephrology and Rheumatology) (Med D)
Wedlich-Söldner, Roland	Institute for Cell Dynamics and Imaging

Applicants from the University of Münster

Pavenstädt, Hermann-Joseph	Medical Clinic of Internal Medicine D (Nephrology and Rheumatology) (Med D)
Wedlich-Söldner, Roland	Institute for Cell Dynamics and Imaging

Projects of the following funding period

CRC 1009 - B10: Calcium mediated actin rearrangement (CAR) in podocyte development and pathogenesis

Duration: 01/07/2020 - 30/06/2024 | 2nd Funding period

Funded by: DFG - Collaborative Research Centre

Type of project: Subproject in DFG-joint project hosted at University of Münster

Related main project

SFB 1009 - Breaking Barriers - Immune cells and pathogens at cell / matrix barriers (SFB 1009/2)

Duration: 01/07/2016 - 30/06/2020 | 2nd Funding period

Funded by: DFG - Collaborative Research Centre

Type of project: Main DFG-project hosted at University of Münster

Publications of the University of Münster resulting from the project

Calcium-mediated actin reset (CaAR) mediates acute cell adaptations

Wales P., Schuberth C., Aufschnaiter R., Fels J., García-Aguilar I., Janning A., Dlugos C., Schäfer-Herte M., Klingner C., Wälte M., Kuhlmann J., Menis E., Kang L., Maier K., Hou W., Russo A., Higgs H., Pavenstädt H., Vogl T., Roth J., Qualmann B., Kessels M., Martin D., Mulder B., Wedlich-Söldner R. (2016)

In: eLife, 5. doi:10.7554/eLife.19850

Research article (journal) | Peer reviewed | Published