FOR 964 - Work package: Function and calcium-dependent regulation of CBL/CIPK-mediated protein phosphorylation

Basic data for this project

Description

The proposed project aims to investigate the contribution of a complex signaling network comprising Calcineurin B-like proteins (CBLs) and their interacting protein kinases (CIPKs) to calcium signaling processes in plants. In general, this project is focused on protein phosphorylation processes as important steps in generating and decoding calcium signals. Specifically this project aims to: investigate protein phosphorylation as functional principle regulating CBL/CIPK complex activity and to investigate differential calcium-dependence of CBL/CIPK complex formation as mechanistic switch in information processing, pursue a reverse genetics analysis of CBL2 and CIPK8 function and identify and characterize CBL/CIPK targets by a combination of two complementary approaches: a yeast two hybrid and BiFC based candidate gene testing approach for ion channels and transporters as well as the non-biased identification of phosphorylation substrates by comparative phospho-proteomics analyses of cbl and cipk mutants.