Mikrobielle Biofabriken: PolyMore - Ein Paenibacillus-Polymyxa-Chassis für die chemische Produktion und neue Sporenprozesse (Teilprojekt A) (PolyMore)

Basic data for this project

Type of project: Participation in federally funded joint project

Duration at the University of Münster: 01/09/2021 - 31/12/2023 (Initial start date: 01/02/2020)

Description

Aim of the project is the development of a novel Paenibacillus polymyxachassis organism for application in biorefinery principles. Based on an already existing tailored CRISPR-Cas9 system this strain will be further developed towards a robust and reliable production host which is perfectly adapted towards the utilization of renewable resources. The genus Paenibacillus is perfectly suited for biorefinery processes by its broad substrate spectra as well as lignin metabolization and manifold antimicrobial activity as well as natural tolerance against inhibitory compounds resulting from lignocellulose-based pretreatments. Within PolyMore we strive for the junction of all benefits from the various Paenibacilli strains in a single highly robust chassis. By use of a holistic systems biology approach we will perform an in-depth characterization which will guide the further optimization.

Keywords: Mikrobiologie; Biofabrik; Bioökonomie; Plattformorganismus

Funding identifier: 031B0855A

Funder / funding scheme:

Federal Ministry of Research, Technology and Space (BMFTR)

Project management at the University of Münster

Schmid, Jochen

Institute of Molecular Microbiology and Biotechnology

Applicants from the University of Münster

Schmid, Jochen

Institute of Molecular Microbiology and Biotechnology

Project partners outside the University of Münster

Max Planck Institute for Terrestrial MicrobiologyGermany
Technical University of Darmstadt (TU Darmstadt)Germany
Norwegian University of Science and Technology (NTNU)Norway

Organisations outside the University of Münster the project was previously carried out

Technical University of Darmstadt (TU Darmstadt)Germany

Publications of the University of Münster resulting from the project

CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa Meliawati, Mweliawati; Teckentrup, Christa; Schmid, Jochen (2022) In: ACS Synthetic Biology, 2022(11 / 1), 77-84. doi:10.1021/acssynbio.1c00565 Research article (journal) \| Peer reviewed \| Published
Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production Meliawati Meliawati, Daniel C. Volke, Pablo I. Nikel, Jochen Schmid (2024) In: Microbial Biotechnology, 17(3), e14438. doi:10.1111/1751-7915.14438 Research article (journal) \| Peer reviewed \| Published
CRISPR-Cas9-Mediated Genome Editing in Paenibacillus polymyxa Ravagnan, Giulia; Meliawati, Mekliawati; Schmid, Jochen (2024) In: Braman, Jeffrey, Carl (eds.), Synthetic Biology, Methods and Protocols , 267-280. New York: Humana Press. doi:10.1007/978-1-0716-3658-9_16 Research article (book contribution) \| Peer reviewed \| Published
Promising non-model microbial cell factories obtained by genome reduction Ravagnan, Giulia; Schmid, Jochen (2024) In: Frontiers in Bioengineering and Biotechnology, 12. doi:10.3389/fbioe.2024.1427248 Review article (journal) \| Peer reviewed \| Published
Implementation of Spore Display in Paenibacillus polymyxa with Different Hydrolytic Enzymes Zander, Maximilian; Schmid, Jochen; Kabisch Johannes (2024) In: Microorganisms, 12(7), 1438. doi:10.3390/microorganisms12071438 Research article (journal) \| Peer reviewed \| Published

CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa Meliawati, Mweliawati; Teckentrup, Christa; Schmid, Jochen (2022) In: ACS Synthetic Biology, 2022(11 / 1), 77-84. doi:10.1021/acssynbio.1c00565 Research article (journal) \| Peer reviewed \| Published
Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production Meliawati Meliawati, Daniel C. Volke, Pablo I. Nikel, Jochen Schmid (2024) In: Microbial Biotechnology, 17(3), e14438. doi:10.1111/1751-7915.14438 Research article (journal) \| Peer reviewed \| Published
CRISPR-Cas9-Mediated Genome Editing in Paenibacillus polymyxa Ravagnan, Giulia; Meliawati, Mekliawati; Schmid, Jochen (2024) In: Braman, Jeffrey, Carl (eds.), Synthetic Biology, Methods and Protocols , 267-280. New York: Humana Press. doi:10.1007/978-1-0716-3658-9_16 Research article (book contribution) \| Peer reviewed \| Published
Promising non-model microbial cell factories obtained by genome reduction Ravagnan, Giulia; Schmid, Jochen (2024) In: Frontiers in Bioengineering and Biotechnology, 12. doi:10.3389/fbioe.2024.1427248 Review article (journal) \| Peer reviewed \| Published
Implementation of Spore Display in Paenibacillus polymyxa with Different Hydrolytic Enzymes Zander, Maximilian; Schmid, Jochen; Kabisch Johannes (2024) In: Microorganisms, 12(7), 1438. doi:10.3390/microorganisms12071438 Research article (journal) \| Peer reviewed \| Published