CRC 1450 - B05: Mechanisms of NET formation during sterile and bacterial inflammation

Basic data for this project

Description

Upon activation at inflammatory sites, neutrophils release extracellular traps (NETs), being beneficial in infectious diseases, but rather detrimental during non-infectious diseases. To investigate this in detail, we will use intravital and ex vivo microscopy, whole-body imaging, and light sheet microscopy to discriminate NET formation in the different compartments of the kidney during sterile inflammation, and of the lung during bacterial infection. To further identify the implication of Pad4, TLR4, and platelets, we will use knock-out or platelet-depleted mice to discriminate their contribution to intra- or extravascular NET formation.