CRC 1450 - A06: Improving intravital microscopy of inflammatory cell response by active motion compensation using controlled adaptive optics

Basic data for this project

Type of project: Subproject in DFG-joint project hosted at University of Münster

Duration at the University of Münster: 01/01/2021 - 31/12/2024 | 1st Funding period

Description

We will advance multiphoton fluorescence microscopy by developing a novel optical module comprised of a high-speed deformable mirror that will actively compensate tissue motion during intravital imaging, for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. To control this module in real-time, we will develop mathematical methods that track and predict tissue deformation. This will allow imaging of inflammatory processes at cellular resolution without mechanical tissue fixation.

Keywords: intravital microscopy; Biophysics; analysis; numerics; Photonics

Website of the project: https://www.uni-muenster.de/CRC-inSight/research/area-A/A06.php

Funding identifier: SFB 1450/1, A06

Funder / funding scheme:

DFG - Collaborative Research Centre (SFB)

Project management at the University of Münster

Wirth, Benedikt

Professorship of Biomedical Computing/Modelling (Prof. Wirth)

Applicants from the University of Münster