Efficient varieties of narrow-leaved lupin (Lupinus angustifolius) for organic farming by exploiting the genetic potential of root traits and rhizosphere interactions (LupiRhiz)

Basic data for this project

Description

Healthy and efficient root systems of the cultivated crops are indispensable prerequisites for reliable and high yields. The project aims to significantly improve the yield and yield stability of narrow-leaved lupins (Lupinus angustifolius) by selecting for root characteristics. In addition to recording the root architecture and root growth rate in rhizotrons, the root chemotype (root exudates and rhizodeposits) and its influence on the rhizosphere, nodule formation and phosphate digestion will be analysed using GC/MS. The genetic basis will be analysed by means of differential expression profiles using RNA-Seq comparisons and quantitative PCR with mRNA. The data will be used for breeding purposes to transfer the root-related traits from bitter forms to low-alkaloid forms of narrow-leaved lupin and combine them with important domestication and quality traits. The domestication traits (e.g. burst resistance, soft-shelledness) are to be tracked and selected using molecular markers. The quality characteristics protein and alkaloid content are recorded via GC/MS and single grain NIRS and included in the selection. In addition, agronomic characteristics such as vigour, stability and late maturity are to be taken into account, which could have a positive effect on future forms of cultivation such as mixed cropping. The project aims to provide novel varieties of narrow-leaved lupin that can open up new cultivation areas and deliver significantly improved grain yields and yield stability via efficient root systems with novel properties. The genetic resources available to the applicant consortium from previous projects and the traits found therein, e.g. for soil pH tolerance, plant architecture and pod set, are ideally suited for this purpose.