Rapid detection and identification of candidemia by direct blood culturing on solid medium by use of lysis-centrifugation method combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)
Idelevich E., Grünastel B., Becker K.
Forschungsartikel (Zeitschrift) | Peer reviewedZusammenfassung
Candida sepsis is a life-threatening condition with increasing prevalence. In this study, direct blood culturing on solid medium using a lysis-centrifugation procedure enabled successful Candida species identification by matrix-assisted laser desorption-ionization time of flight mass spectrometry on average 3.8 h (Sabouraud agar) or 7.4 h (chocolate agar) before the positivity signal for control samples in Bactec mycosis-IC/F or Bactec Plus aerobic/F bottles, respectively. Direct culturing on solid medium accelerated candidemia diagnostics compared to that with automated broth-based systems.
Details zur Publikation
Fachzeitschrift: Journal of Clinical Microbiology (J Clin Microbiol)
Jahrgang / Bandnr. / Volume: 55
Ausgabe / Heftnr. / Issue: 1
Seitenbereich: 97-100
Status: Veröffentlicht
Veröffentlichungsjahr: 2017
Sprache, in der die Publikation verfasst ist: Englisch
DOI: 10.1128/JCM.01787-16
Link zum Volltext: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85008395922&origin=inward
Stichwörter: Blood culture; Candida; Candidemia; Direct blood culturing; Lysis-centrifugation; MALDI-TOF; Mass spectrometry; Rapid tests; Sepsis
Autor*innen der Universität Münster
| Becker, Karsten | Institut für Medizinische Mikrobiologie |
| Idelevich, Evgeny | Institut für Medizinische Mikrobiologie |