Investigating the adduct formation of organic mercury species with carbonic anhydrase and hemoglobin from human red blood cell hemolysate by means of LC/ESI-TOF-MS and LC/ICP-MS

Hogeback J, Schwarzer M , Wehe CA , Sperling M, Karst U

Forschungsartikel (Zeitschrift) | Peer reviewed

Zusammenfassung

The interaction of mercury species with human erythrocytes is studied to investigate possible high molecular binding partners for mercury species. Human blood hemolysate was spiked with methylmercury and investigated by means of liquid chromatography (LC) coupled to electrospray ionization time of flight mass spectrometry (ESI-ToF-MS) and inductively coupled plasma mass spectrometry (ICP-MS). Beside adduct formation of mercury species with hemoglobin, the main compound of the erythrocytes, mercury binding to the enzyme carbonic anhydrase was revealed. Due to an enzymatic digest of the protein-mercury adduct, the binding site at the free thiol group of the protein was identified. These results indicate that carbonic anhydrase might play a role in mercury toxicity.

Details zur Publikation

FachzeitschriftMetallomics
Jahrgang / Bandnr. / Volume8
Ausgabe / Heftnr. / Issue1
Seitenbereich101-107
StatusVeröffentlicht
Veröffentlichungsjahr2016
Sprache, in der die Publikation verfasst istEnglisch
DOI10.1039/c5mt00186b
StichwörterHUMAN WHOLE-BLOOD; MASS-SPECTROMETRY; IN-VITRO; ICP-MS; NUCLEOTIDE-SEQUENCE; METHYLMERCURY; THIMEROSAL; BINDING; HPLC; ERYTHROCYTES

Autor*innen der Universität Münster

Hogeback, Jens
Professur für Analytische Chemie (Prof. Karst)
Karst, Uwe
Professur für Analytische Chemie (Prof. Karst)
Schwarzer, Miriam
Institut für Anorganische und Analytische Chemie
Sperling, Michael
Professur für Analytische Chemie (Prof. Karst)
Wehe, Christoph
Professur für Analytische Chemie (Prof. Karst)