Association of Shiga toxin glycosphingolipid receptors with membrane microdomains of toxin-sensitive lymphoid and myeloid cells.

Kouzel IU, Pohlentz G, Storck W, Radamm L, Hoffmann P, Bielaszewska M, Bauwens A, Cichon C, Schmidt MA, Mormann M, Karch H, Müthing J

Forschungsartikel (Zeitschrift) | Peer reviewed

Zusammenfassung

Glycosphingolipids (GSLs) of the globo-series constitute specific receptors for Shiga toxins (Stxs) released by certain types of pathogenic Escherichia coli strains. Stx-loaded leukocytes may act as transporter cells in the blood and transfer the toxin to endothelial target cells. Therefore, we performed a thorough investigation on the expression of globo-series GSLs in serum-free cultivated Raji and Jurkat cells, representing B- and T-lymphocyte descendants, respectively, as well as THP-1 and HL-60 cells of the monocyte and granulocyte lineage, respectively. The presence of Stx-receptors in GSL preparations of Raji and THP-1 cells and the absence in Jurkat and HL-60 cells revealed high compliance of solid-phase immunodetection assays with the expression profiles of receptor-related glycosyltransferases, performed by qRT-PCR analysis, and Stx2-caused cellular damage. Canonical microdomain association of Stx GSL receptors, sphingomyelin, and cholesterol in membranes of Raji and THP-1 cells was assessed by comparative analysis of detergent-resistant membrane (DRM) and nonDRM fractions obtained by density gradient centrifugation and showed high correlation based on nonparametric statistical analysis. Our comprehensive study on the expression of Stx-receptors and their subcellular distribution provides the basis for exploring the functional role of lipid raft-associated Stx-receptors in cells of leukocyte origin.

Details zur Publikation

FachzeitschriftJournal of Lipid Research (J Lipid Res)
Jahrgang / Bandnr. / Volume54
Ausgabe / Heftnr. / Issue3
Seitenbereich692-710
StatusVeröffentlicht
Veröffentlichungsjahr2013
Sprache, in der die Publikation verfasst istEnglisch
DOI10.1194/jlr.M031781

Autor*innen der Universität Münster

Cichon, Christoph
Institut für Infektiologie
Karch, Helge
Institut für Hygiene
Mormann, Michael
Institut für Hygiene
Müthing, Johannes
Institut für Hygiene
Schmidt, M. Alexander
Institut für Infektiologie