The Calcineurin B-like calcium sensors CBL1 and CBL9 together with their interacting protein kinase CIPK26 regulate the Arabidopsis NADPH oxidase RBOHF.

Drerup MM, Schlücking K, Hashimoto K, Manishankar P, Steinhorst L, Kuchitsu K, Kudla J

Forschungsartikel (Zeitschrift) | Peer reviewed

Zusammenfassung

Stimulus-specific accumulation of second messengers like reactive oxygen species (ROS) and Ca(2+) are central to many signaling and regulation processes in plants. However, mechanisms that govern the reciprocal interrelation of Ca(2+) and ROS signaling are only beginning to emerge. NADPH oxidases of the respiratory burst oxidase homolog (RBOH) family are critical components contributing to the generation of ROS while Calcineurin B-like (CBL) Ca(2+) sensor proteins together with their interacting kinases (CIPKs) have been shown to function in many Ca(2+)- signaling processes. In this study, we identify direct functional interactions between both signaling systems. We report that the CBL-interacting protein kinase CIPK26 specifically interacts with the N-terminal domain of RBOHF in yeast two-hybrid analyses and with the full-length RBOHF protein in plant cells. In addition, CIPK26 phosphorylates RBOHF in vitro and co-expression of either CBL1 or CBL9 with CIPK26 strongly enhances ROS production by RBOHF in HEK293T cells. Together, these findings identify a direct interconnection between CBL-CIPK-mediated Ca(2+) signaling and ROS signaling in plants and provide evidence for a synergistic activation of the NADPH oxidase RBOHF by direct Ca(2+)-binding to its EF-hands and Ca(2+)-induced phosphorylation by CBL1/9-CIPK26 complexes.

Details zur Publikation

FachzeitschriftMolecular Plant
Jahrgang / Bandnr. / Volume6
Ausgabe / Heftnr. / Issue2
Seitenbereich559-569
StatusVeröffentlicht
Veröffentlichungsjahr2013 (30.03.2013)
Sprache, in der die Publikation verfasst istEnglisch
DOI10.1093/mp/sst009

Autor*innen der Universität Münster

Drerup, Maria
Molecular Genetics and Cell Biology of Plants (AG Prof. Kudla)
Hashimoto, Kenji
Molecular Genetics and Cell Biology of Plants (AG Prof. Kudla)
Kudla, Jörg
Molecular Genetics and Cell Biology of Plants (AG Prof. Kudla)
Manishankar, Prabha
Molecular Genetics and Cell Biology of Plants (AG Prof. Kudla)
Schlücking, Kathrin
Molecular Genetics and Cell Biology of Plants (AG Prof. Kudla)
Steinhorst, Leonie
Molecular Genetics and Cell Biology of Plants (AG Prof. Kudla)