qPCR and mtDNA SNP analysis of experimentally degraded hair samples and its application in forensic casework.
Köhnemann S, Pennekamp P, Schmidt PF, Pfeiffer H
Forschungsartikel (Zeitschrift)Zusammenfassung
A mitochondrial DNA (mtDNA) quantification PCR (qPCR) method was developed and applied in a study with experimentally degraded hair samples (first study) and in a criminal case (second study). In the first study, the amount of detectable mtDNA decreased drastically after an incubation time of 1 month on a moist tissue in a heating cabin at 37 degrees C. In the second study, when the qPCR assay showed positive quantification results, further analysis of 32 mtDNA single-nucleotide polymorphisms (SNPs) via SNaPshot technique was always possible, indicating that successful mtDNA SNP analysis of forensic samples can be guaranteed by pre-screening samples with the qPCR described here.
Details zur Publikation
Fachzeitschrift: International Journal of Legal Medicine (Int J Legal Med)
Jahrgang / Bandnr. / Volume: 124
Ausgabe / Heftnr. / Issue: 4
Seitenbereich: 337-342
Status: Veröffentlicht
Veröffentlichungsjahr: 2010
Sprache, in der die Publikation verfasst ist: Englisch
Stichwörter: Hair Color; Polymorphism Single Nucleotide; DNA Mitochondrial; Humans; Middle Aged; Specimen Handling; DNA Fingerprinting; Microscopy Electron Scanning; Adult; Female; Polymerase Chain Reaction; Hair; Hair Color; Polymorphism Single Nucleotide; DNA Mitochondrial; Humans; Middle Aged; Specimen Handling; DNA Fingerprinting; Microscopy Electron Scanning; Adult; Female; Polymerase Chain Reaction; Hair
Autor*innen der Universität Münster
| Köhnemann, Stephan | Institut für Rechtsmedizin |
| Pennekamp, Petra | Klinik für Medizinische Genetik |
| Pfeiffer, Heidi | Institut für Rechtsmedizin |